MegaELISA® / ELISA

The Enzyme-linked immunosorbent assay (ELISA) is a common method basing on an enzymatic colour reaction. It is used to detect proteins (e.g. antibodies), viruses or low-molecular compounds (e.g. hormones, inflammation markers, toxins) in the sample.

For the test, the highly specific antibody-antigen interaction is utilised.

Most used diagnostic formats are the indirect ELISA for the detection of antibodies in the sample and the Sandwich ELISA.

In the indirect ELISA, the antigen is bound to the plate. This antigen binds the specific antibodies in the sample. In the Sandwich ELISA, an analyte-specific antibody is bound to the plate which binds the analyte in the sample.

The detection is done in both cases by a specific enzyme marked detection antibody (conjugate). The coupled enzyme changes the colour of the specific substrate into a coloured product.