MegaFLUO® / IFT
Indirect Immunofluorescence test
In the indirect immunofluorescence test (IFT), specific antibodies present in the prediluted sera bind to the antigens present on the slide. Non-bound unspecific serum proteins are washed off. Fluorescein-marked FLUO FITC antibodies in the conjugate bind to the antigen-antibody complexes. Evaluation is done with a fluorescence microscope (filter system for FITC) with 400× magnification.