Focused on Veterinary Diagnostics
Focused on Veterinary Diagnostics

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  • > FASTest® C. diff 2T

    Applicable to

    Pig, Dog, Cat, Horse

    FASTest® C. diff 2T
    > Field of Application

    FASTest® C. diff 2T is a rapid immunochromatographic test for the qualitative detection of Glutamate Dehydrogenase (GDH) and of Toxins A/B from Clostridioides difficile in feces of dog, cat, horse and pig.

    > General

    Clostridioides difficile is a gram positive anaerobic spore former. It causes diarrhoea in various species. Studies prove the incidence of C. difficile in animal food. Therefore, a zoonotic potential for humans (diarrhoea, colitis) must be implied. Additionally, there are hints of mutual transfer between dog/cat and human within a household.

    Most important virulence factors for the development of C. difficile infection (CDI) are the enterotoxin A (TcdA) and cytotoxin B (TcdB).

    Dog/cat: C. difficile can be proven in feces of healthy juvenile and adult animals as well as in animals with diarrhoea (single animals, nosocomial infections in animal hospitals and shelters). A significant correlation between C. difficile and diarrhoea could not be proven, but feces samples of animals with diarrhoea showed significantly higher TcdA (increased secretion of liquid into the intestinal lumen) and/or TcdB (lethal damage of the intestinal wall) detection as with healthy animals.

    Horse: Both in single animals and with diarrhoea outbreaks in herds CDI (TcdA & TcdB) occur, especially in foals, partly associated with C. perfringens, then mostly with deathly course within 3 days. Clinically indicative are colic, partly without/before diarrhoea onset and massive antibiotic associated colitis.

    Pig: In 1–7 days old piglets, CDI is one of the most important diarrhoea diseases (mortality up to 16%). The prevalence decreases with increasing age. The fecal-oral colonization with C. difficile happens in endemic areas at 100% within 48h, lactogenic via the sow (ca. 25%) or aerogenic via surroundings. Clinical symptoms (yellow-watery diarrhoea, but also constipation) are not always visible. Risk factors for development of an acute CDI are age, provocation dose, but also associated toxins and the administration of antibiotics. Retarded growth, lower weaning weight and severe economical losses are the consequences.

    Diagnosis of an acute CDI can be difficult due to the endemic nature of C. difficile. With a two-step diagnostic of GDH (Glutamate Dehydrogenase) and the Toxins A/B, the proof can succeed with high certainty. The proof of GDH is said to be very sensitive compared to culture (golden standard) and therefore can be used as so-called “exclusion test”. On the other hand, the proof of the Toxins A/B is seen as highly specific (but less sensitive) compared to culture. Therefore, the double test can be optimally used as confirmation test.

    In combination with anamnesis and clinic, the FASTest® C. diff 2T is suitable as an on-site diagnostic test for the secure exclusion or proof of a C. difficile infection.


    Detection of


    Sample material:


    Test time/duration

    15 minutes


    Room temperature (15–25°C)


    GDH: 96%, Toxin A/B: 99%


    GDH: 98%, Toxin A/B: 96.3%

    Comparison method

    GDH: ELISA, Toxin A/B: Cytotoxicity

    Test procedure

    Rapid test

    Tab-Container Desktop


    Art.-No. 796010RG1

    request> Into the basket


    1 test-kit contains 10 revolver test tubes with 2 dipsticks each