13.08.2015 - New: MegaPCR® for Veterinarian and Laboratory
With its new product line MegaPCR® VET, MEGACOR Diagnostik GmbH extends its veterinary medicinal in vitro diagnostics range. Herewith, veterinarians and laboratories are steeled for future challenges in veterinary diagnostics.
- Test-kit for POCKIT™ device, ready-to-use
- The compact PCR for beginners
- Veterinary practice / veterinary hospital
- Veterinary laboratory without experience in PCR
- Test-kit for all established PCR laboratory cyclers
- taco™ extraction device: fully automated DNA purification
- PetNAD™ extraction kit: manual DNA purification
MegaPCR® VET are validated test-kits that can be handled on all common cyclers as well as the newly developed POCKIT™. The POCKIT™ is based on iiPCR and allows PCR newcomers to perform the PCR on-site within one hour. The attractively priced POCKIT™ is suitable for both: laboratories as well as veterinarians, and it also withstands the examination by OIE.
Thereby, both laboratories as well as veterinarians take the lead in diagnostics.
If you have further questions, do not hesitate to contact us! www.megacor.com
Flyer MegaPCR® VET
29.07.2015 - Correlation between leucocyte number and CRP value
A study with 924 dogs revealed no correlation between the leucocyte number and the C-reactive protein (CRP). The leucocyte number can be influenced by various pathologic and physiologic factors, so that with acute inflammation increased CRP values can be seen both with physiological and pathological leucocyte numbers (leucocytosis / leucopenia). On the contrary, the value of CRP concentration reflects the intensity of inflammation as well as its persistence.
Through its low physiological basal concentration, its rapid increase (10-100 fold) already 4h after beginning of the inflammatory stimulus, its peak concentration after 24 h as well as its decrease 48 h after the end of the inflammatory stimulus, the CRP determination as marker of an inflammatory event is a valuable diagnostic component in daily routine.
The FASTest® CRP canine enables the veterinarian to have a simple, rapid and on-site decision whether an inflammatory process is going on or not. Therefore, the initiation of further diagnostic or therapeutic measures on-site is possible.
Kleintierpraxis 60, Heft 7 (2015): Untersuchungen zur CRP-Konzentration bei verschiedenen Leukozytenkonzentrationen: D.Breu, S.Klenner, E. Müller: S. 349-357.
17.04.2015 - FASTest® CRYPTO Strip
A study with 120 snake feces samples of various species showed that the presence of reptile specific Cryptosporidia is rather marginal. More often, C. parvum (zoonosis) and C. muris (feeding with infected prey) were detected.
To exclude possible consequences for human health, reptile stocks should be tested regularly for Cryptosporidia, especially Cryptosporidium parvum.
Source: Kleintierpraxis 60, Heft 4 (2015)
Cryptosporidium bei Heimtierschlangen in Italien: Molekulare Charakterisierung und zoonotische Konsequenzen; Vet Parasitol 197 (1-2): 68-73
12.02.2015 - Giardia duodenalis in dog and cat
Another actual study* confirms the suspicion that dogs and cats are carriers of the human pathogen genotypes (assemblages) A and B.
Therefore, a Giardia infection in dog and cat must be seen as potential zoonotic. To prove the infectious cysts, in principle dogs with intermittent or chronic diarrhoea symptoms should be tested according to the capc.vet guide lines. Due to intermittent cyst shedding, a single negative test result does not exclude a persistent Giardia infection. Hence, with current diarrhoea symptoms and negative test result, a test repetition ca. 1 week later with a fecal sample from 3 consecutive days or of three sequential feces sample is reasonable. Only a repeatedly negative test result can exclude a Giardia infection definitely.
*Literature: V34 Giardia duodenalis bei Hunden und Katzen: eine epidemiologische Studie; S. Rehbein, C. Klotz, E. Müller, A. Aebischer, B. Kohn; 23. Jahrestagung der FG „Innere Medizin und klinische Labordiagnostik“ der DVG (InnLab), 23./24. Januar 2015 in Leipzig, Schattauer Tierärztliche Praxis Kleintiere 1/2015
06.02.2015 - Titre Check before CPV vaccination via FASTest® CPV Ab
Does your patient have a good immunity (adequate antibody titre) against the Canine Parvovirus? From which titre before vaccination, a booster vaccination makes sense?
Although an actual study shows that the German dog population is protected well against Parvovirus, however, many dogs do not react with an appropriate antibody increase after vaccination. Dogs showing a non-protective antibody titre during titre check do react significantly more frequently with an adequate titre increase.
To guarantee an adequate titre increase after vaccination, the verification of the antibody status is highly recommended.
Literature: V35 Prävalenz von Antikörpern gegen kanine Parvoviren und Reaktion auf Wiederholungsimpfung bei gesunden Hunden: M. Riedl, U. Truyen, K. Hartmann; 23. Jahrestagung der FG „Innere Medizin und klinische Labordiagnostik“ der DVG (InnLab), 23./24. Januar 2015 in Leipzig, Schattauer Tierärztliche Praxis Kleintiere 1/2015
03.02.2015 - Diarrhoea in the cat
Despite of the main appearance of Giardia duodenalis single infections (FASTest® GIARDIA Strip), co-infections with Tritrichomonas foetus (InPouchTM TF-Feline) are not seldom.
Therefore, especially in case of "treatment failure for Giardia", an additional tritrichomonads diagnostics with InPouchTM TF-Feline should be done to verify an infection with T. foetus.
Literatur: P03 Giardia duodenalis – Koinfektionen mit Tritrichomonas foetus bei Katzen: J. Csokai, E. M. Sicking, E. Müller; 23. Jahrestagung der FG „Innere Medizin und klinische Labordiagnostik“ der DVG (InnLab), 23./24. Januar 2015 in Leipzig, Schattauer Tierärztliche Praxis Kleintiere 2/2015
15.11.2014 - paraTEST® VET - Flotation diagnosis system
paraTEST® VET is a simple, hygienic and standardised parasite diagnosis system for the microscopical proof of parasite eggs and oocysts from the feces of pocket pets, pets and farm animals.
The flotation method is a long-standing coproscopical detection method in the veterinary medicine. The paraTEST® VET system allows the uptake of an adequate amount of feces with help of the blue sieve insert, its hygienical keeping in the paraTEST® VET analysis box as well as, if needed, the transport into the veterinary laboratory. By addition of the flotation solution with high specifical weight, the parasite eggs searched for flotate to the surface of the forming convex meniscus and settle to the bottom of the cover slip on the meniscus after ca. 15 minutes. This cover slip can then be analysed with a microscope by trained personnel for the presence of parasite eggs.
paraTEST® VET is therefore perfectly suitable for the on-site parasite diagnosis. The rapid detection of the various parasite stadiums gives the veterinarian the possibility to initiate therapeutic and prophylactic measurements as soon as possible.
Further information here: paraTEST® VET
Instructions for use
16.08.2014 - Study: FASTest® NEOSPORA caninum
MEGACOR Diagnostik GmbH has developed a rapid test for the detection of anti-Neospora caninumNeospora caninum antibodies in whole blood, serum or plasma of dogs and cattle. The test is based on an immunochromatographic reaction, where Neospora caninum antibodies present in the sample are bound to membrane fixed, recombinant Neospora caninum antigens. The interpretation is qualitative. A control line appears confirming the test functionality in principle. In case of a positive sample, an additional Neospora caninum-specific test line appears on the test membrane.
The Institute of Parasitology of Vetsuisse Faculty Bern, Länggass-Str. 122, 3001 Bern was authorized by MEGACOR to run a sensitivity testing of the veterinary test named above. The study contains following issues:
- Check-up of the provided FASTest® NEOSPORA caninum test-kits
- Results of sensitivity testing
- Summary and discussion
Summary and discussion
FASTest® NEOSPORA caninum test-kit is clearly designed. Test procedure is easy and time saving. The test results are qualitative. A quantification of results is not possible. Reading of the test result should be strictly after 15 minutes, due to increasing unspecific reactions between 15 and 20 minutes, respectively, and increase of results that are more difficult to interpret.
In view of the reference lab for neosporosis, FASTest® NEOSPORA caninum test-kit complies to all requirements for a sensitive (relative sensitivity of 96.2%) and specific (relative specificity of 100%) rapid test for the detection of N. caninum in dog and cattle. Unspecific reactions should be clarified with a complementary examination (IFAT, ELISA).
The complete text of the study can be read here.
23.06.2014 - New LEPTOSPIRA IgM antibody rapid test for the dog
New MEGACOR LEPTOSPIRA IgM antibody rapid test
FASTest® LEPTOSPIRA IgM ad us. vet. is an immunochromatographic rapid test for the qualitative detection of Leptospira spp. antibodies in whole blood, plasma or serum of the dog.
Due to the non-existence of a cross immunity of the vaccination serovars and an increasing “serovar shift”, leptospirosis becomes more important (world-wide increasing prevalences especially for L. icterohaemorrhagica, L. canicola, L. grippotyphosa, L. australis and L. pomona).
Incubation time as well as symptoms are strongly dependent on age, immune status and serovar type. Subclinical infections are common and proven by numerous studies proving a higher seroprevalence than the prevalence of the clinical infection.
The actual method of reference, the microagglutination test (MAT), can differentiate between single serovars but has a strongly varying sensitivity (30–80%) depending on infection stadium and does not distinguish between IgM and/or IgG antibodies. Therefore, in the acute phase of leptospirosis a pure IgM proof is a significant diagnostic value.
The FASTest® LEPTOSPIRA IgM is an important diagnostic tool for the veterinarian for the fast and simple on-site detection of a leptospirosis in the early state of infection. Therefore, further laboratory and therapeutic as well as prophylactic measures (zoonosis) can be started immediately.
20.05.2014 - New parasite flotation system: OVATEC® Plus
New distribution product
OVATEC® Plus is a simple, clean and professional fecal testing system with a high accuracy and safety for the identification of parasitic eggs of common gastrointestinal parasites.
The OVATEC® Plus System consists of a zinc sulfate flotation medium which floats parasitic ova (eggs) as well as Giardia cysts, an optimised filter device for improved detection of even low-level ova burdens and an overflow reservoir for the reduction of exposure to potentially hazardous samples.
The OVATEC® Plus System is a client-friendly collection procedure with an easy-to-follow instruction for use and a test device that seals tightly for transport.
28.04.2014 - FASTest® CRYPTO-ROTA D2T
MEGACOR DIAGNOSTIK GmbH expands its FASTest® product portfolio with the rapid test FASTest® CRYPTO-ROTA D2T.
By means of only one test FASTest® CRYPTO-ROTA D2T, the veterinarian is able to aetiologically detect Cryptosporidium parvum and Rotavirus antigens simultaneously in feces of cattle, horse, pig, cat and dog.
Clinically, both diseases cannot be distinguished, and their high infectiosity, the increasing number of co-infections and their high zoonotic potential for humans are reason enough to give an aetiological proof of the pathogen by modern on-site coprodiagnostics.
The FASTest® CRYPTO-ROTA D2T is, similar to the single tests FASTest® CRYPTO Strip and FASTest® ROTA Strip, simple, fast with a test interpretation after 5 minutes and has high sensitivity (C. parvum 94.1% and Rotavirus 96%) and specificity values (C. parvum 95.5% and Rotavirus 100%).
Instructions for use
27.10.2013 - New TOXOPLASMA antibody rapid test for cat & dog
Toxoplasma gondii, agent of toxoplasmosis, plays an important epidemiologic role especially in the cat, but also in the dog. Definite hosts (gamogony and oocysts production) are only cats and other felids (especially lynx, ozelot, puma). The oocysts excreted by the definite host are potentially infectious for humans (zoonosis). Dogs are only intermediate hosts and therefore do not egest oocysts.
Infected animals should be detected, because they are a potential source of infection for humans. Even though only 0.6–1.4 % of cat feces samples in Germany contain oocysts, ca. 70 % of the cats are infected with Toxoplasma.
Approximately 2 weeks post infection, the antibody titre increases (seroconversion) with maximum IgG titres between 2–4 weeks (optimal time point of testing). Thereafter, the titre decreases onto an “infestation titre” (< 1:50), the antibodies can persist. The prevalence for seropositivity (Germany ca. 50–55 %) increases with advanced age.
Clinical symptoms are not pathognomonic in dog and cat and depend on age and immune status of the animal. Seriously acute courses with encephalitis, enteritis, hepatitis, myositis and cell lysis during protozoan propagation mostly lead to death, especially in puppies. In older animals with chronic progression, anorexy, lethargy, fever as well as damage of myocard, liver and CNS are main issues. Main issues in the dog are central nervous disorders ranging from slight malfunction to epileptiform failure.
Because a combination of coprologic and serologic detection (2x at an interval of 14 days) allows direct conclusions on the infection potential of the cat, the veterinarian using FASTest® TOXOPLASMA g is able to identify fast, simple and on-site the T. gondii status of the suspicious animal. This allows to immediately start further diagnostic and therapeutic as well as prophylactic measurements.
Due to similar symptoms of Neosporosis infection, especially young dogs should be differential diagnostically tested with FASTest® NEOSPORA caninum.
Instructions for use
02.08.2013 - New ANAPLASMA antibody rapid test for dog & horse
Anaplasmosis („granulocytotropic ehrlichiosis“) is a bacterial-borne infectious disease vectored by ticks. The agent Anaplasma phagocytophilum plays a future epidemiologic role especially in the dog and in the horse.
As a tick-transmitted (especially Ixodes spp.; Dermacentor spp.) bacterial infectious disease, tick territories (endemic area) should be considered as potential breeding ground for dog and horse. Single infections are usually subclinical and self-limiting. Coinfections are common and can aggravate the course of disease significantly. Here, especially Borrelia infections must be mentioned, particularly as they are transmitted by the same tick species.
Clinical symptoms are fever, apathy, stiff muscle, polyarthritis with joint pain / swelling and lameness as well as weight loss, thrombocytopenia, anaemia, petechial haemorrhages and increasing inflammatory values (CRP, haptoglobin). Subarachnoid haemorrhage could lead to central nervous disorders. Additionally, horses can show metabolic acidosis, oedema, laminitis as well as coffee brown urine.
Antibody titres increase in the first 2–3 weeks post infection and can decrease below the detection limit after 7 months.
Due to similar clinical symptoms in tick-transmitted infectious diseases, the veterinarian using FASTest® ANAPLASMA is able to identify on-site the A. phagocytophilum / A. platys antibody status of the suspicious animal. This enables to start immediately further diagnostic investigations (IFAT, blood smear / count, PCR etc.) as well as prophylactic and therapeutic measurements.
Flyer FASTest ANAPLASMA
08.07.2013 - New: FASTest NEOSPORA caninum is now applicable for dog and cattle
New MEGACOR FASTest for Neospora antibodies for DOG & CATTLE
FASTest® NEOSPORA caninum ad us. vet. is an immunochromatographic rapid test for the qualitative detection of anti-Neospora caninum antibodies in whole blood, plasma or serum of the dog and cattle.
Neospora caninum plays an important epidemiologic role in dogs and cattle.The dog (esp. watch dogs, stray dogs) is a definitive host (intestinal neosporosis) and excretes infectious oocysts with the feces. The intermediate host, especially cattle, but also goats, sheep and horse, gets infected via grazing land and / or water contaminated with oocysts (horizontal direct / exogeneous infection) and / or intrauterine via already infected mothers (vertical indirect / endogeneous infection). Both dogs (systemic neosporosis with neuromuscular disorders, myocarditis, hepatitis, pneumonia, dermatitis) and cattle (accumulating abortions in all states of gestation, dead births and weak calves) can develop a clinical disease.
Due to the short excretion period and the low amount of oocysts in dog feces, the detection of antibodies using FASTest® NEOSPORA caninum becomes very important for the diagnosis of neosporosis. Due to horizontal, but particularly to the economical considerably important vertical placental transmission of infection onto the offspring, suspicious cattle stocks should be tested for antibodies against N. caninum using FASTest® NEOSPORA caninum.
16.05.2013 - New: FASTest IgG bovine
New MEGACOR cattle IgG rapid test
FASTest® IgG bovine is an immunochromatographic fast test for the semiquantitative detection of IgG antibodies (immune status) in native blood, whole blood, plasma and serum of cattle.
The immune globulines G (IgG, gamma globulines) belong the the antibody class G mainly directed against viruses and bacteria.
Due to the special placenta conditions, newborn calves show no to hardly noteworthy IgG (especially IgG1) and often show an immune deficiency syndrome (IDS). Therefore, the most important base for an immunoprophylaxis is an adequate supplementation with IgG.
Also, in cows the content of IgG economically plays an important role. If the intrapartal IgG concentration is already significantly decreased (< 15 mg / ml), an increased incidence of genital associated diseases (dystocia, metritis, mastidis a.s.o.) will be seen in the peripartal period.
The optimal test time slot using FASTest® IgG bovine is between 24 to 48 h (max up to 7 days) post natum in calves and from 3rd to 7th day post partum in cows.
FASTest® IgG bovine enables the veterinarian on-farm and withoud any technical costs to confirm (FASTest® IgG bovine: IgG ≤ 12 mg / ml) or to exclude (FASTest® IgG bovine: IgG > 12 mg / ml) a suspicion for FPT / IDS in the cow as well as in the calf.
For further information please click here.