Simple practical test to detect or exclude FIP infection

 

The final diagnosis of FIP (Feline Infectious Peritonitis) poses a major diagnostic challenge to the veterinarian. Between diverse methods for the detection of FIP infection, the RIVALTA test is regarded as an important component for the differentiation of transudate (non-inflammable excretion of fluid in body cavities) and exudate (usually inflammatory excretion of fluid in body cavities). Cats suspicious for FIP or with effusion symptoms of unknown origin should be principally aspirated and tested by means of RIVALTA FIP-VETube.

When the exudate suspicious for FIP does contain protein, the punctate drop precipitates during plunging into the aqua-acetic acid mixture. This can be observed by the characteristic formation of more or less stable precipitation products or cloudy mists, forming drops or smears. In contrast, the punctate drop being transudate completely dissolves during sinking in the aqua-acetic acid mixture.
Being fast, simple and reliable, usable on-site and highly significant, the RIVALTA FIP-VETube is an ideal tool to prove or exclude a FIP infection.

The compact test boxes (10 tests per box) are storable at room temperature (15-25° C) for 24 months after production.

For a fast, competent and reliable technical support and for orders, please contact info@megacor.at.

Neu FASTest® D4T bovine ad us.vet.

Testkit for the detection of Cryptosporidium parvum, bovine Coronavirus (BCV), E. coli-K99 (F5) and Rotavirus in feces of bovines

Diarrhea caused by bovine Coronavirus (BCV), Cryptosporidium parvum (C. parvum), enterotoxic Escherichia coli K99 (F5) and Rotavirus (RV), is a major cause of severe illness and death in calf rearing. Especially calves within the first two weeks of life (neonatal diarrhea, ND, scours) diarrhea occurs often with high mortality!
In this case aetologic diagnosis of all bovines regardless of which age (asymptomatic shedders) and a check-up of feeding and keeping management. Cryptospiridiose is a special disease of the ND complex because of its specific therapy. Therefore early diagnostic is an assumption for a successful therapy because medication has to begin within 24 hours after onset of diarrhea! For this an early detection is necessary for a successful treatment as well as a fast beginning of prophylactical measures.

The test procedure and test interpretation of FASTest® D4T bovine is most user-friendly due to an innovative „revolver-test tube"(R) loaded with 4 test membranes.
All 4 test membranes were incubated in the same buffer dilution and at the same time. The test interpretation of all 4 membranes occurs without any contact with potential infectious diarrhea agents while rotating slowly the revolver-test tube (R).

Sample material:
- Feces from bovines

Easy test procedure: 4 membranes at the same time using one Puffer
- pulpy-compact feces 1 (one) respectively watery feces 2 (two) coated spoons

Fast test interpretation after 5 minutes:

- NEGATIVE: 1 Line at „C" = Control line
- POSITIVE: 1 Line at „C" and 1 Line at „T" = Test line

High test reliability:
- FASTest® D4T bovine BCV Sensitivity (88,9%) & Specificity (98,7%)
- FASTest® D4T bovine E.coli (F5) Sensitivity (90%) & Specificity (98,5%)
- FASTest® D4T bovine Crypto Sensitivity (94,1%) & Specificity (95,5%)
- FASTest® D4T bovine ROTA Sensitivity (96%) & Specificity (100%)

The compact Test-kits (à 10 tests) have a long shelf life of 18 months from manufacturing and can be stored at room temperature (15-25°C).

MEGACOR Diagnostik GmbH
Lochauer Str. 2 
A-6912 Hörbranz 
AUSTRIA 

	Study	download
	Article	download
	Fieldstudy	download

New FASTest® CRP canine ad us.vet.

Test-kit for the detection of C-reactive-Protein (CRP) in Dogs anticoagulated Whole Blood, Serum or Plasma

Acute-phase-Proteins become more and more important in the early diagnosis of inflammatory processes (Infections, Immune disorders, Neoplasia and traumata, aso).

Several studies identify canine serum C-Reactive-Protein (CRP) in the dog as the most sensitive acute-phase protein because of its fast concentration increase after 6-10h from the beginning of inflammatory processes and because of its higher sensitivity in comparison to the increase of leucocytes.
For this reason the detection of CRP is a well established laboratory parameter for the diagnosis and monitoring of inflammatory processes.

An actual comparison study (Tridelta CRP ELISA - FASTest® CRP canine) from the University of Berlin* evaluates FASTest® CRP canine as an adequate on-site screening test in the routine veterinary clinic and a good diagnostic tool for monitoring of inflammatory processes.

Optional sample material:
- Anti coagulated whole blood (10 µl), Plasma or Serum (5 µl each)

Simple test procedure:
- 5 / 10 µl sample volume + 2 drops buffer solution

Fast test evaluation after exactly 5 minutes:
- Negative: < 5 mg/l und Positive: ≥ 5 mg/l .

High reliability:
- Serum/Plasma: Sensitivity (96%) & Specificity (81%)
- Whole blood: Sensitivity (95%) & Specificity (94%)

The compact Test-kits (à 2/10/25 tests) have a long shelf life of 18 months from manufacturing and can be stored at room temperature (15-25°C).

*Study H.Plickert, B.Kohn, G. Arndt, L. Brunnberg, R. EInspanier, 2010
Klinik und Poliklinik für kleine Haustiere, Freie Universität Berlin, Institut für Biometrie und Informationsverarbeitung und Institut für Veterinär-Biochemie, Freie Universität Berlin :
Evaluierung eines CRP-Schnelltests für die tierärztliche Praxis, Vortrag DVG Tagung Düsseldorf, 2010

MEGACOR Diagnostik GmbH

Lochauer Str. 2

A-6912 Hörbranz

AUSTRIA

	Vergleichsstudie ELISA-Schnelltest	download

New FASTest® BOR in TICK ad us.vet.

Testkit for the detection of Borrelia Antigens in the tick

In Europe Lyme-Borreliosis, is caused by the bacteria Borrelia burgdorferi-sensu-lato (B.b.s.l.)-complex. At dogs or at humans they could be transmitted by several tick species, especially by the castor bean tick "Ixodes ricinus". From 12 hours after tick bite the B.b.s.l subspecies (especially B. burgdorferi sensu stricto, B. afzelii, B. garinii) were transmitted by the salivary gland into the stab wound. Peak levels of Borrelia excretion would be reached approximately 72 h after tick bite.

If a tick does or doesn´t contain Borrelia antigen depends strongly from seasonal and geographic factors. Studies showed prevalences up to 50% in endemic areas. Using FASTest® BOR in TICK potential Borrelia antigens in ticks could be detected fast, simple and reliable.

Normally the Borreliosis diagnostic could be very difficult because of the possible uncharacteristically symptoms (neurological, dermatological, cardiac and rheumatoid manifestations). Because of this the early diagnosis using FASTest® BOR in TICK is a very important diagnostic tool and time marker. In case of a positive test result diagnostic (mainly Borrelia antibody status at the time of tick bite), therapeutic and prophylactic measures could be initiated immediately!

Sample material:
- Ticks of any size

Simple and hygienic test procedure:
- Squeeze tick + 4 Drops buffer diluent
- Therefrom drop 3 Drops into the SAMPLE window of the cassette

Fast test evaluation after 10 minutes:
NEGATIVE: 1 Line at „C" = CONTROLline
POSITIVE: 1 Line at „C" and 1 Line at „T" = TESTline

High reliability::
- Sensitivity (78,6 %) & Specificity (98,2 %)

The compact Test-kits (à 1/5 tests) have a long shelf life of 18 months from manufacturing and can be stored at room temperature (15-25°C).

MEGACOR Diagnostik GmbH

Lochauer Str. 2

A-6912 Hörbranz

AUSTRIA

New FASTest® LH ad us.vet.

Screening-Testkit for the qualitative Detection of luteinizing Hormon (LH) in Serum of Dog and Cat

The detection of luteinizing Hormon (LH) in Serum of Dog and Cat become more and more important in the reproduction diagnosis.

Mainly for dogs the need for an assortative mating (natural mating or artificial insemination), increase of conception rate, gestation diagnosis or prognosis of whelping date increases.

Thus the focus concentrates to an exact termination of the mating time. Because the detection of LH is the exactest method for the detection of the LH-Peak, ovulation time and mating time as well as of the whelping date, FASTest® LH is an optimal diagnostic tool to come up to the requirements of the modern reproductive medicine.

Further FASTest® LH is suitable to differentiate between fertile (intact ovar / testes) and infertile (ovar-/ orichiectomized resp. chemical castrated) dogs and cats. Primarily for animals with unknown fertility status FASTest® LH is a fast, simple and reliable tool to get detailled information about the gonadal status.

Sample material:
- Serum

Simple test procedure:
- 3 Drops sample volume - no need for buffer diluent!

Fast test evaluation after 20 minutes
Comparison of colour intensity of TESTline and CONTROLline

NEGATIVE: Testline "T" < Controlline „C"
POSITIVE: Testline "T" ≥ Controlline „C"

The compact Test-kits (à 2/10/25 tests) have a long shelf life of 18 months from manufacturing and can be stored at room temperature (15-25°C).

MEGACOR Diagnostik GmbH

Lochauer Str. 2

A-6912 Hörbranz

AUSTRIA

NEW evaluated NEW - MEGABlot® IgG-BORRELIA canis and MEGABlot® IgM-BORRELIA canis - NEW evaluated NEW

Immunoblot for the Detection of IgG or IgM - Antibodies against Borrelia burgdorferi sensu lato (B. b. sensu stricto, afzelii und garinii) in Serum and Plasma of the dog

Diagnosis of Borreliosis is often difficult, and must be based on history, clinical  manifestations (e.g. lethargy, fatigue, fever followed by shifting leg lameness and arthritis and laboratory serological tests. The standard approach diagnosing an  ongoing Borreliosis involves an in-clinic screening using "first step" rapid technique (e.g. FASTest® LYME). In case of a positive test result a "second step" test like an ELISA (MegaELISA® IgG Borrelia canis), IFAT (MegaScreen® FLUOBORRELIA c and/or a Western Blot (MEGABlot® IgG-BORRELIA canis) must be done for  confirmation! Known to be gold standard confirmatory test Western Blot (MEGABlot® IgG-BORRELIA canis) is a perfect tool to get a reliable estimation about the Borreliosis status of the dog resulting in a definitive diagnosis. The Western Blot technology is extremely useful in dissecting the IgM/IgG immune response to Borrelia infections which develops gradually over a period of weeks to years and which involves the appearance of IgM and IgG antibodies directed against a number of Borrelia-associated proteins.

The interpretation of the MegaBlot® IgG/IgM-BORRELIA strip result is done by using a test-kit-specific Template consisting of a developed antigen strip cut from the same membrane used to prepare the Blot- antigen strips. This template strip has been exposed to a positive control serum in order to exhibit bands representative for a Lyme infection. Special immunodominant bands (p100 (93 kDa), p 58 (58kDa) flagellin (41 kDa), BmpA (39 kDa), OspA (32.5 kDa), OspC (22-23 kDa) and p18 (18 kDa) were scored using a newly adapted point scoring system* listed on the right side of the Template.

The MegaBlot® IgG/IgM -BORRELIA canis was proofed* against recomBlot Borrelia canis IgG/IgM as an economic, qualified and reliable tool for laboratory needs.

KEY POINTS OF THE STUDY:

► No single specific band, not even VlsE could differentiate between vaccinated or infected dogs!

► Score system of MegaBlot® IgG/IgM -BORRELIA canis roules out that single specific bands like (p 100; p39; OspC) could make a false positive Blot result!

►No single Western blot test containing VlsE or not could differentiate between vaccinated or infected dogs!

►Repeating Western blots after the onset of clinical symptoms are recommended in finding the definitive diagnosis!

 Indications for a postvaccinal immune response
- Stable high OspA
- Decrease of band intensities: p 100, Osp C

 Strong Indications for a ongoing immune response after natural infection
- Stable or rising band intensities: p 100, p39, Osp C

 

*Humoral Immune Response in Dogs Naturally Infected with Borrelia burgdorferi Sensu Lato and in Dogs after Immunization with a Borrelia Vaccine conducted by University of Veterinary Vienna , Department for Companion Animals and Horses; 1.Medical Clinic for Internal Medicine and Infectious Diseases, Dr. M. W. Leschnik. et al CLINICAL AND VACCINE IMMUNOLOGY, May 2010, p.828-835; Vol 17, No.5

	Leschnik et al (2010)	download

NEU FASTest® CRYPTO STRIP ad us. vet. für KALB & IGEL NEU

Der FASTest® CRYPTO Strip erweitert seinen Zulassungsbereich (FLI-B 500) auf den Igel (Prävalenz 26,6 % im Frühjahr bis zu 36,6 % im Herbst/Winter). Der immunochromatographische Schnelltest weist C. parvum-Oberflächenantigene (Oozysten u. vegetative C. parvum-Formen, sowie deren Zerfallsprodukte) innerhalb von 5 Minuten im Kot nach.

Durch seine hohe Spezifität (99,9%) und Sensitivität (96,7%) eignet er sich zur ätiologischen Vor-Ort-Diagnostik bei klinischem Verdacht (Igel/Kalb) sowie zum Screening beim Bestandsproblem „Neonatale Kälberdiarrhoe“.

Die hohen MEGACOR-Produktstandards (Haltbarkeit 18 Monate, variable Testkit-Formate (2 od. 10 Tests), Lagerung bei Raumtemperatur, sowie optimaler Liefer- und Beratungsservice) gewährleisten seinen einfachen, schnellen und zuverlässigen Einsatz in der Veterinärdiagnostik.

New InPouchTM TF – Feline

Detection of TRITRICHOMONAS FOETUS

Tritrichomonas foetus, well known since many years as a venereal infection in cattle causing infertility, abortion and endometritis, play also an important role in cat`s diarrhea.

T. foetus is a single-celled flagellated protozoa, located especially in the large bowel (colon) and causes colitis and chronic diarrhea especially in puppies, young cats and pedigree cats (multi cat household).

Prevalence ranges between 20-25% Co-infections with other feline enteric infections like Giardiasis or Cryptosporidiosis are often, so testing for Giardiasis and Cryptosporidiosis (e.g. FASTestTM GIARDIA Strip or. FASTestTM CRYPTO Strip). is recommendable!

A rectal swab or a swab from fresh feces (0,03g) is sufficient to start culturing T. f. trophozoits using a high selective and high sensitive InPouchTM TF-Feline – Culture medium. Already an inoculum (0,03g) containing as little as 1 T. foetus trophozoits is sufficient to potentially result in a presumptive positive test

Using QUICK – DIAGNOSTIC (concentration/sedimentation of trophozoits) motile T. foetus –Trophozoits could be detected at the earliest 15 minutes after inoculation of the InPouchTM TF-Feline – Culture medium. If QUICK DIAGNOSTIC shows no trophozoits CULTURE – DIAGNOSTIC (Incubation of inoculated InPouchTM TF-Feline at 35 – 37 °C for 18-24 hours) is recommended.

Compact Test-kits (packed with 5/20/100 Tests) could be stored at room temperature. Shelf life is 12 month from manufacturing date.

If a special technical and scientific assistance is needed or in case of an order please send us an e-mail: info@megacor.at

Untersuchung verschiedener Verfahren zum Nachweis von Cryptosporidiose beim igel

In den Igelstationen in Deutschland treten häufiger antibiotika-und antiparastika resistente Durchfälle auf, die in einigen Fällen auch zum Tod der Tiere führen. Bei einer weitergehenden Kotuntersuchung eines Igels mit therapieresistentem Durchfall konnten Kryptosporidienoozysten nachgewiesen und der Igel erfolgreich behandelt werden.

Ziel unserer orientierenden Studie war es, die Prävalenz der Kryptosporidiose beim Braunbrustigel (Erinaceuseuropaeus) zu untersuchen und gleichzeitig verschiedene Nachweisverfahren im Hinblick auf Sensitivität und leichte Durchführbarkeit in den Igelstationen zu testen.

	Cryptosporidiose	download

Comparison of different methods diagnosing Cryptosporidiosis in the european hedgehog (Erinaceus europaeus)

Y. Kuhnert, B. Bangoura, K. Ditmar, F. Stöckel, U. Seewald und R. Schmäschke Institute of Parasitology, Veterinary School of Leipzig, Germany

The occurrence of cryptosporidiosis is rarely presented in the literature. Most reviews about hedgehogs suffering from parasites lack of attention to cryptosporidiosis. Main reason for this could be the fact, that hedgehog feces not usually are screened for cryptosporidiosis. In cooperation with the association “Pro Igel (Hedgehog) e.V.” basic investigations of the incidence of cryptosporidiosis in the european hedgehog (Erinaceus europaeus) were done in germany by the Institute of Parasitology, Veterinary School of Leipzig. Concurrently 5 commercial test-methods diagnosing cryptosporidiosis were compared: Heine-staining; Ziehl-Neelsen-staining; ELISA, IFAT and FASTest® CRYPTO Strip, whereas Ziehl- Neelsen Staining and ELISA was only proofed using positive feces samples.

Considering the results all different test methods are basically correspondent. Especially the rapid test (FASTest® CRYPTO Strip) is qualified best for the detection of cryptosporidiosis due to it`s easy practicability and interpretation, mainly for the staff of hedgehog care centers.

To verify these basic investigations, especially the prevalence of cryptosporidiosis in the hedgehog, more investigations would be necessary in autumn due to the fact that more feces samples from young hedgehogs which are thought to be higher infected with cryptosporidiosis could be obtained.